XP® Monoclonal Antibodies and XMT® Technology
The XP® Benefit
XP® monoclonal antibodies are a line of high quality rabbit monoclonal antibodies exclusively available from Cell Signaling Technology. Any product labeled with XP® has been carefully selected based on superior performance in the most relevant applications.
XP® monoclonal antibodies are generated using XMT®technology, a proprietary monoclonal method developed at Cell Signaling Technology. The technology provides access to a broad range of antibody-producing B cells unattainable with traditional monoclonal technologies, allowing more comprehensive screening and the identification of XP®monoclonal antibodies with:
- eXceptional Specificity
- Case Study 1: SpecificityAs with all of our antibodies, the antibody is specific to your target of interest, saving you valuable time and resources.
- eXceptional Sensitivity
- Case Study 2: SensitivityThe antibody will provide a stronger signal for your target protein in cells and tissues, allowing you to monitor expression of low levels of endogenous proteins, saving you valuable materials.
- eXceptional Stability and Reproducibility
- Case Study 3: ReproducibilityXMT technology combined with our stringent quality control ensures maximum lot-to-lot consistency and the most reproducible results.
- eXceptional Performance
- Case Study 4: Overall PerformanceXMT technology coupled with our extensive antibody validation and stringent quality control delivers XP®monoclonal antibodies with eXceptional Performance in the widest range of applications.
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377
Figure 1. Confocal immunofluorescent analysis of HeLa cells using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Figure 2. Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D2C8) XP®Rabbit mAb #3377 versus propidium iodide (DNA content). The boxed population indicates Phospho-Histone H3 (Ser10) positive cells.
EGF Receptor (D38B1) XP® Rabbit mAb #4267
Figure 3A. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using EGF Receptor (D38B1) XP® Rabbit mAb #4267.
Figure 3B. Immunohistochemical analysis of paraffin-embedded human placenta using EGF Receptor (D38B1) XP® Rabbit mAb #4267.
Figure 4. Confocal immunofluorescent analysis of A-549 cells, untreated (left) or treated with Epidermal Growth Factor (Human EGF) (right), using EGF Receptor (D38B1) XP® Rabbit mAb #4267 (green). Blue pseudocolor = DRAQ5® #4084(fluorescent DNA dye).
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511
Figure 5. Confocal immunofluorescent analysis of COS cells, untreated (left) or anisomycin-treated (right), usingPhospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Figure 6. Western blot analysis of extracts from COS and 293 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (upper) orp38 MAPK Antibody #9212 (lower).
