Biological imaging: Beyond fluorescence

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Nature 467, 407-408 (23 September 2010) | doi:10.1038/467407a; Published online 22 September 2010

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Biological imaging: Beyond fluorescence

Bruce E. Cohen¹

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Abstract

Nanoparticles that generate light through a mechanism known as second harmonic generation have been used to image live tissue. The particles overcome many problems associated with fluorescent probes for bioimaging.

Biologists love optical microscopy. Open any cell-biology journal and you will be struck by a collection of colourful microscopy images worthy of a Seurat retrospective, with fluorescent staining patterns that help to unravel the complexities of cell signalling and development, neuroscience and cancer.

1. Bruce E. Cohen is at the Molecular