Gateway® Cloning
Gateway® recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system. Widely adopted in the research community with more than 1,500 references since its launch, Gateway® technology makes collaboration across research disciplines easy and convenient and enables access to a multitude of vectors from these research groups for truly multidisciplinary scientific studies.
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Gateway® Cloning Technology
Finally, new advancements such as MultiSite Gateway® technology makes Invitrogen’s Gateway® cloning the ideal cloning method for protein expression and functional analysis.
- Fast, one hour, room temperature cloning reactions with >99% efficiency deliver the clone you need
- Maintain orientation and reading frame without using restriction enzymes or ligation makes expression-ready clones
- Eliminate resequencing ensures consistent results throughout your experiment using the same clone from target identification to validation
- Shuttle insert DNA from one expression vector to another affords flexibility while simplifying your cloning workflow
Learn more about Gateway® cloning technology
Entry Vectors & Kits
pENTR vectors are available with efficient Directional TOPO® cloning and classic restriction enzyme and ligase cloning.
Not sure which Gateway® Entry vector to use?
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